Iterative l ‐Tryptophan Methylation in Psilocybe Evolved by Subdomain Duplication

Biotransformation of tryptophan by liquid medium culture of Psilocybe coprophila (Basidiomycetes).

Chemical reactions performed by fungi have been used as a modern tool in chemistry. In this work, we show the tryptophan biotransformation with Psilocybe coprophila on liquid culture medium. The results prove once more the versatility of fungi in performing a wide range of industrially attractive chemical reactions.

Induction by L-tryptophan and an analogue, alpha-methyl-DL-tryptophan, of the enzymes catabolizing L-tryptophan in Pseudomonas.

An investigation was made of the pattern of induction of the enzymes that metabolize l-tryptophan through kynurenic acid (the quinoline pathway) in Pseudomonas fluorescens. The first four enzymes in the pathway were not induced in the same proportions or in the same time courses. This lack of coordinate induction excludes a mechanism of regulation of these enzymes at a single site as proposed i...

l-Tryptophan Production by Achromobacter liquidum.

Conditions for the production of tryptophanase from Achromobacter liquidum and for the conversion of l-serine and indole to l-tryptophan were studied. The enzyme could be produced in amounts as great as 0.750 U/ml (degradation) and 0.294 U/ml (synthesis) by shaking cultures at 30 degrees C in a medium containing dextrin, yeast extract, l-tryptophan, and l-glutamic acid. l-Tryptophan was produce...

FixJ-regulated genes evolved through promoter duplication in Sinorhizobium meliloti.

The FixLJ two-component system of Sinorhizobium meliloti is a global regulator, turning on nitrogen-fixation genes in microaerobiosis. Up to now, nifA and fixK were the only genes known to be directly regulated by FixJ. We used a genomic SELEX approach in order to isolate new FixJ targets in the genome. This led to the identification of 22 FixJ binding sites, including the known sites in the fi...

Iterative Prototype Optimisation with Evolved Improvement Steps